Identification of clinical Streptococcus pneumoniae isolates among 1 other alpha and nonhemolytic streptococci using the VITEK MS 2 MALDI - TOF mass spectrometry system 3 4

نویسندگان

  • Christine SEGONDS
  • Marie-Françoise PRERE
چکیده

4 Damien DUBOIS, Christine SEGONDS, Marie-Françoise PRERE, Nicole 5 MARTY, Eric OSWALD 6 7 Laboratoire de Bactériologie-Hygiène, Institut Fédératif de Biologie, Centre Hospitalo8 Universitaire, Toulouse, F-31059, France 9 Université Paul Sabatier-Toulouse III, Toulouse, F-31076, France 10 11 Running title: S. pneumoniae identification with MALDI-TOF VITEK MS 12 13 * Corresponding author. 14 Mailing address: Laboratoire de Bactériologie-Hygiène hospitalière, Hôpital Purpan, Institut 15 Fédératif de Biologie (IFB), 330, avenue de Grande-Bretagne, TSA 40031, 31059 Toulouse 16 cedex 9, France. 17 Phone: 33 5 67 69 03 94. 18 Fax: 33 5 67 69 04 83. 19 E-mail: [email protected] 20 21 $ These authors have contributed equally to this work. 22 23 Copyright © 2013, American Society for Microbiology. All Rights Reserved. J. Clin. Microbiol. doi:10.1128/JCM.03069-12 JCM Accepts, published online ahead of print on 10 April 2013

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Identification of clinical Streptococcus pneumoniae isolates among other alpha and nonhemolytic streptococci by use of the Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry system.

Discrimination between Streptococcus pneumoniae and its close relatives of the viridans group is a common difficulty in matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry-based identification. In the present study, the performances of the Vitek MS MALDI-TOF mass spectrometry system were assessed using 334 pneumococci, 166 other S. mitis group streptococci, ...

متن کامل

Using Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) Complemented with Selected 16S rRNA and gyrB Genes Sequencing to Practically Identify Clinical Important Viridans Group Streptococci (VGS)

There are challenges in viridans group streptococci (VGS) identification especially for the mitis group. Few studies have investigated the performance of MALDI-TOF MS system in VGS identification. Using 16S rRNA gene and gyrB gene sequencing as a gold standard, the performance of two MALDI-TOF MS instruments in the identification of 181 VGS clinical isolates was studied. The Bruker Biotyper and...

متن کامل

Viridans Group Streptococci Clinical Isolates: MALDI-TOF Mass Spectrometry versus Gene Sequence-Based Identification

Viridans Group Streptococci (VGS) species-level identification is fundamental for patients management. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been used for VGS identification but discrimination within the Mitis group resulted difficult. In this study, VGS identifications with two MALDI-TOF instruments, the Biotyper (Bruker) and the VITEK ...

متن کامل

Multicenter evaluation of the Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry system for identification of Gram-positive aerobic bacteria.

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF) is gaining momentum as a tool for bacterial identification in the clinical microbiology laboratory. Compared with conventional methods, this technology can more readily and conveniently identify a wide range of organisms. Here, we report the findings from a multicenter study to evaluate the Vitek MS v2.0 sy...

متن کامل

MALDI-TOF mass spectrometry proteomic based identification of clinical bacterial isolates

BACKGROUND & OBJECTIVES Pathogenic bacteria often cause life threatening infections especially in immunocompromised individuals. Therefore, rapid and reliable species identification is essential for a successful treatment and disease management. We evaluated a rapid, proteomic based technique for identification of clinical bacterial isolates by protein profiling using matrix-assisted laser deso...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2013